Fibroblast growth factors (FGFs), a heparin binding growth factor, exhibit widespread mitogenic and neurotrophic activities in a variety of different cells including mesenchymal, neuroectodermal and endothelial cells.
aFGF (FGF-1) and bFGF (FGF-2) are present in relatively high levels in CNS.
aFGF is expressed by a subset of neuronal populations, while bFGF is expressed by astrocytes, both lack signal peptides.
Human bFGF is a 17.2kD protein containing 155 amino acid residues.
The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro.
There are differences in the tissue distribution and concentration of these 2 growth factors.
Subunit: Monomer.
Interacts with CSPG4 and FGFBP1.
Found in a complex with FGFBP1, FGF1 and FGF2.
This protein binds heparin more strongly than does aFGF.
Belongs to the heparin-binding growth factors family.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:2000Immunohistochemistry: 1:1000-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：sheep
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitute in 100ul of sterile ddH2O.
Specificity：Recognizes human Basic Fibroblast Growth Factor. Species Crossreactivity: mouse and rat
