CYR61 is a cysteine-rich, heparin-binding, growth factor belonging to the CCN family of secreted regulatory proteins.
It stimulates chondrogensis and angiogenesis, playing an important role in cartilage and bone formation, as well as tumor growth and vascularization.
Applications:Suitable for use in Western Blot, Immunofluorescence, Immunocytochemistry and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (Paraffin): 1:100 Western Blot (ECL): 1:200.
A band can be seen at ~37kD representing the processed form of CYR61 protein
Immunofluorescence (IC): 1:10-1:500Optimal dilutions to be determined by the researcher.
Positive Control:MDA-MB-231 breast cancer cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:Recognizes human CYR61.
Isotype:IgG
Calc Applications Abbrev:IC IF IHC WB
Calc Crossreactivity:Hu
Immunogen:Synthetic peptide corresponding to the human CYR61 protein sequence (between residues 150-250). Species Sequence Homology: rat and mouse, 88%.