84-1099-90　Cre (Cre Recombinase, Cre (Cyclization Recombination) Enzyme) 50ul　C7909-02

特徴

• The Cre (cyclization recombination) enzyme, a member of a large family of recombinases, recognizes loxP which is a sequence motif of 34bp from the PI bacteriophage.
• If a DNA seqment is flanked by two loxP sites in the same orientation, Cre excises that segment leaving a single loxP site in the remaining DNA molecule.
• The excised DNA segment is subsequently degraded.
• Cre and lox comprise the Cre/lox system which was first developed in the late 1980’s to artificially manipulate gene expression.
• As an example, mice with the Cre protein expressed in a specific cell type are bred with mice that contain a target gene surrounded by loxP sites.
• When the mice are bred, the cells carrying Cre will cause those cells to lose the target gene.
• If the Cre gene is, for example, bound to a promoter that only allows Cre production in neuronal cells, the target gene will be deleted only in those cells.
• Since 1989 the Cre/lox system has been used extensively and there are numerous animal, plant and bacterial stocks that already contain the Cre gene driven by ubiquitous, tissue-specific or inducible promoters and provide a quick method for breeding experiments.
• The Cre/lox system has the advantage of working in almost any type of cell.
• This has led to its application in many experiments, for example in selectively labeling neuronal cells in the brain thereby differentiating them from other types of surrounding cells.
• Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000-1:2000.
• Detects a band at ~36-38kD Immunoprecipitation: 1:50-1:200Immunohistochemistry (Paraffin): 1:1000-1:5000Immunohistochemistry: Frozen sectionsOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.