Cytochrome C Oxidase (COX), which is located in the inner mitochondrial membrane, is the terminal enzyme complex of the mitochondrial electron transport chain.
It collects electrons that are transferred from reduced Cytochrome C and donates them to molecular oxygen, which is then reduced to water.
Antibodies to this protein make an effective loading control for mitochondria.
COXIV (COX4 )is generally expressed at a consistently high level.
However, be aware that many proteins run at the same molecular weight as COXIV.
Some caution is required when using antibodies to this protein as a loading control, as COXIV expression can vary under some manipulations.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris-glycine, 150mM sodium chloride, pH 7.2.
Specificity：This antibody is specific for COX41. Species Crossreactivity: This antibody reacts with the human COX41 protein.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：A synthetic peptide made to an internal region of the human COX41 protein (within residues 1-100). [Swiss-Prot# P13073]. Homology: 100% human, 99% mouse and cow, 92% monkey, 90% rat, and 76% Zebrafish
