One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
A connexon is composed of a hexamer of connexins.
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:10,000-1:20,000Western Blot: 1:20,000-1:50,000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months after receipt at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：sheep
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.
Specificity：Recognizes human Connexin 45 at 47kD. Band is abolished by preincubation with the immunizing peptide. Does not react in Western Blot with membranes from non-transfected cells or cells transfected with cDNA encoding Connexin-37 or Connexin-40.
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu
Immunogen：A synthetic peptide (QAYSHQN NPHGPRE) as part of human Connexin-45 protein (aa354-367) conjugated to diphtheria toxoid. Cellular Localization: Membrane; multi-pass membrane protein. Belongs to the connexin family, alpha-type (group II) subfamily.
