These antibodies are well suited to detect extracellular matrix proteins in normal as well as disease state tissues.
Disruption of tissue organization is the hallmark of neoplasia.
Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture.
Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur.
Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunofluorescence/Immunocytochemistry and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:ELISA: 1:5000-1:50,000Immunohistochemistry (Paraffin): 1:50-1:200Immunoprecipitation: 1:100Western Blot: 1:1000-1:10,000 Immunofluorescence (IC): 1:10-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human Collagen Type III. Species Crossreactivity: rat, bovine, mouse, feline, sheep and most mammals. Typically <1% cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. Has negligible cross-reactivity with Type I, II, IV, V or VI collagens. Non-specific cross-reaction with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Isotype：IgG
Calc Applications Abbrev：E IC IF IHC IP WB
Calc Crossreactivity：Bo Fe Hu Ma Rt Sh
Immunogen：Collagen Type III from human and bovine placenta. Cellular Localization: Extracellular matrix
