Note=Cytoplasmic face of coated pits and vesicles.
PTM: The N-terminus is blocked.
Belongs to the clathrin heavy chain family.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ul
Host:rabbit
Source Antibody:rat
Grade:Serum
Purity:Serum
Form:Supplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.
Specificity:This antibody has been shown to be specific for clathrin. Species Crossreactivity: Rat, other species have not yet tested.
Isotype:IgG
Calc Applications Abbrev:IHC WB
Calc Crossreactivity:Rt
Immunogen:A synthetic peptide (KAGLLQRALEHFTDLYDIKR) corresponding to the amino acids 619-638 of rat clathrin conjugated to diphtheria toxin has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from clathrin protein in mouse, human and bovine.