DNA fragmentation is one of the critical steps in apoptosis, which is induced by DNA fragmentation factor (DFF).
DFF is composed of two subunits, a 40kD caspase-activated nuclease (DFF40) and a 45kD inhibitor (DFF45).
Recently a novel family of cell-death-inducing DFF45-like effectors (CIDEs) has been identified.
Human CIDE-3 is a novel member of CIDEs.
There are 2 transcripts, CIDE-3 and CIDE-3alpha, were present in HepG2 and A375 cells.
Consistent with its chromosome localization at 3p25, a region associated with high frequency loss of heterozygosity in many tumors, CIDE-3 may play an important role in prevention of tumorigenesis.
Applications:Suitable for use in Western Blot and Immunocytochemistry/Immunofluorescence.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Positive Control: CIDEC Overexpression Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a llquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human CIDE-3.
Isotype：IgG
Calc Applications Abbrev：IC IF WB
Calc Crossreactivity：Hu
Immunogen：Residues 10-25 LLYPKSLSRHVSVRTS] of the 27 kDa human CIDE-3 protein. Homology to FSP27, which is the mouse homolog of CIDE-3: 93% sequence identity.
