84-1099-58　Carbonic Anhydrase IX (CA9) 100ul　C1105-80E

特徴

• Carbonic anhydrase (CA) is an enzyme that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions.
• It is abundant in all mammalian tissues.
• Because of its functionality, it has become an important diagnostic marker for various cancers, most notably renal cell carcinoma (RCC).
• There are many genes that are inducible by hypoxia, via HIF-1 alpha.
• CA IX is one of the most inducible genes because of its stability and location within the membrane.
• Carbonic anhydrases have a widespread role in regulating pH in normal tissues, by regulating hydrogen ion (H+) flux.
• The pH is important in cell death under hypoxia, thus a blockade of CA IX results in increased cell death under hypoxia.
• Therefore, CA IX has become a reliable histochemical marker of hypoxia.
• Applications:Suitable for use in Immunofluorescence/Immunocytochemistry, Western Blot, Flow Cytometry and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Immunofluorescence (IC): 1:1000 Western Blot: 1:2000Flow Cytometry: 1:1000Immunohistochemistry (Frozen, paraffin): 1:1000.
• Optimal dilutions to be determined by the researcher.
• Positive Control:Renal carcinoma
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.