The Capsaicin Receptor (VR1, vanilloid receptor 1) is an ion channel activated by capsaicin, the pungent ingredient in hot peppers, and by noxious thermal heat.
It is expressed by and isolated from nociceptive sensory neurons.
Sequence analysis suggests that VR1 has transmembrane domains and intracellular N- and C-termini.
It shares structural similarities with the family of putative store-operated calcium channels.
It is thought to function as a transducer of noxious thermal stimuli and have involvement in inflammatory pain.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: 1:1000-1:2000 using frozen sections of rat dorsal root ganglia (DRG) and spinal cord.
The rat tissue was prepared by perfusion fixation with 4% formaldehyde, 10% saturated picric acid in buffered saline.
Frozen sections (40um) were used and the secondary antibody was conjugated with HRPOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitute with 100ul of sterile ddH2O.
Specificity：Recognizes human Capsaicin Receptor. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：IHC
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to aa608-621, PSESTSHRWRGPA of human capsaicin receptor, conjugated to KLH.
