Calnexin is a calcium-binding protein which interacts with newly synthesized glycoproteins in ER for facilitating protein assembly and/or the retention within ER of unassembled protein subunits as well as incorrectly folded proteins thereby playing a key role in ER's quality control apparatus.
The specificity of calnexin for glycoproteins is defined by a lectin site, which binds an early oligosaccharide intermediate on the folding glycoprotein.
When associated with partial T-cell antigen receptor complexes which escape immature thymocytes's ER, calnexin regulates thymocyte maturation process and is also implicated in receptor-mediated endocytosis at synapses.
MAPK3/ERK1 phosphorylates calnexin at Ser-565 leading to increased calnexin-ribosomal association and DHHC6 mediated calnexin palmitoylation leads to its preferential localization to perinuclear rough ER.
Palmitoylation also facilitate calnexin association with RTC/ribosome-translocon complex that is essential to efficient folding of glycosylated proteins.
Applications:Suitable for use in Immunocytochemistry/Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunocytochemistry/Immunofluorescence: 1:100Western Blot: 1:1000Immunoprecipitation: 1:100Immunohistochemistry (Paraffin): 1:100, using antigen retrieval with sodium citrate buffer (pH 6.0)
Optimal dilutions to be determined by the researcher.
Positive Control:HeLa cells, HeLa whole cell lysate, Mouse bladder tissue.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid (serum) in PBS, containing 50% glycerol aand sodium azide.
Specificity：Recognizes human Calnexin at ~90 kD (actual weight: 97kD). Species Crossreactivity: mouse. Cellular Localization: Endoplasmic reticulum.
Isotype：IgG
Calc Applications Abbrev：IC IHC IP WB
Calc Crossreactivity：Hu Mo
Immunogen：A synthetic peptide corresponding to a portion of the C-terminal between aa550-591 of human Calnexin protein (P35565). Species Sequence Homology: Xenopus, canine, orangutan, rat, bovine, porcine, chicken, Drosophilia, guinea pig, quail, rabbit, sheep 100%.
