The Bmi1 oncogene has been shown to induce telomerase activity and immortalize human mammary epithelial cells.
Bmi1 extends the replicative life span of human fibroblasts by suppressing the p16-dependent senescence pathway.
Bmi1 is also important in maintaining stem cells' ability to self renew.
It has also been termed an oncogene with aberrant expression in various carcinomas.
It has been shown to be a negative regulator of p19Arf, Ink4a (p14Arf) and tp53 known tumor repressors.
Ablation of Bmi1 is required for short term survival of cancer cells.
Positive Control: Human and mouse skeletal muscle
Applications:Suitable for use in Western Blot, Immunocytochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: A band is seen at ~37kD.
Some less intense non-specific bands are also present
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris-glycine, 150mM sodium chloride, pH 7.2, 0.05% sodium azide.
Specificity：Recognizes human Bmi-1. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：IC IF WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide made to an internal region (within residues 1-100) of the human Bmi1 protein. [Swiss-Prot# P35226]. Cellular Localization: Nuclear
