Biotin is used as a growth factor in mammalian cell culture as well as having numerous immunological purification roles in avidin/streptavidin-biotin binding mechanisms.
Applications:Suitable for use in Immunoelectrophoresis, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, F.I.T., FLISA and Flow Cytometry/FACS.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:20-1:2000Immunocytochemistry: 1:50-1:5000F.I.T.: Mean fluorescence intensity of a 1:1000 dilution at Ex-488nm (+/-9)/Em-518nm (+/-9) = 6643 MFI’sFLISA: Mean fluorescent value of 8ug/ml of FITC conjugate when tested against biotinylated Chicken IgG/Y coated at 4ug/ml = 1558 MFI’sOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：goat
Grade：Affinity Purified
Purity：Affinity purified using solid phase biotin. ~95% based on SDS-PAGE.
Form：Supplied as a in 10mM sodium phosphate, 0.15M sodium chloride, pH 7.2, 1% BSA (protease/IgG free), 0.05% sodium azide. Labeled to fluorescein isothiocyanate (FITC)
Specificity：Recognizes free Biotin and Biotin coupled to BSA, KLH or IgG/Y. Two predominant bands are seen at 50 and 24kD in SDS-PAGE. Does not react with free BSA or free KLH.
