The INK4a-ARF locus is comprised of two tumor suppressors, p16INK4a and p14ARF.
These two proteins are encoded through differential splicing of alternative first exons.
The p16INK4a (exon 1alpha) protein inhibits the cyclin D-dependent kinases (CDK) that control the phosphorylation of the Rb protein and cell proliferation.
The p14ARF gene product complexes with the MDM2 protein within the nucleus, thus modulating the activity of the p53 protein.
P14ARF is a potent tumor suppressor in the presence of wild-type p53, while mutant p53 substantially reduces growth inhibition by p14ARF.
Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2500ELISA: 1:100-1:2000Immunohistochemistry (paraffin): 1:100-1:500Optimal dilutions to be determined by the researcher.
Positive Control:BT549 or HeLa whole cell extracts
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris-Citrate/Phosphate, pH 7.0-8.0, 0.09% sodium azide.
Specificity：Recognizes human p14ARF. The epitope recognized by this antibody maps to a region between residue 82 and C-terminus (aa132) of human p14ARF using the numbering given in entry Uniprot ID Q8N726.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corrresponding to human p14ARF, at C-terminal. Species sequence homology: primate, Orangutan and White-tufted-ear marmoset.
