84-1099-14　ATP7b (PWD, WC1, WND) 100ul　A3999-82A

特徴

• ATP7b is an important protein for copper transport and elimination of excess copper from the body.
• ATP7b transports metals in and out of cells using ATP.
• There are 3 known isoforms of the ATP7b gene; A is found in the liver, kidney, and brain, the shorter form B is found in brain tissue, and the third isoform, known as WND/140kD is found in mitochondria.
• Mutations in the ATP7b gene can cause Wilson’s disease, an inherited disorder causing copper poisoning in the brain and liver.
• Applications:Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry (Paraffin): 1:500Western Blot: 1:1000.
• Band at ~165kDImmunocytochemistry: 1:500Optimal dilutions to be determined by the researcher.
• Positive Control:HuH7 hepatocarcinoma cell extracts
• Cellular Localization:Golgi apparatus; Predominantly found in trans-Golgi network membrane; multi-pass membrane protein (By similarity).
• Isoform 2: Cytoplasm.
• The cleaved form WND/140kD is located in mitochondria.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.