Applications:Suitable for use in ELISA, Immunofluorescence, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:100-1:3000Western Blot: 1:100-1:3000Immunohistochemistry: 1:100-1:3000Optimal dilutions to be determined by the researcher.
Note: Pre-autophagosomal and other perivacuolar punctate structures.
The proper trafficking of ATG9 between the pre-autophagosomal structure and the other punctate structures requires ATG2, ATG18, ATG23, the ATG1-ATG13 complex and the phosphatidylinositol 3-kinase complex I.
Belongs to the ATG9 family.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.
Specificity：IHC, WB and ELISA confirmed the specificity for ATG9A (APG9L1) . Species Crossreactivity: Human, rat. Other species not yet tested.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to the C-terminal of human APG9A (APG9L1). The peptide is homologous with the corresponding sequence derived from APG9A (APG9L1) protein in mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and Canis familiaris (dog).
