Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I.
A subpopulation of form I is subsequently converted to a smaller form (form II).
Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
ENZYME REGULATION: Inhibited by N-ethylmaleimide.
Cellular Localization:Cytoplasm (Probable).
Tissue Specificity:Highly expressed in skeletal muscle, heart, liver and testis.
Belongs to the peptidase C54 family.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.
Specificity：Recognizes human ATG4C. Species Crossreactivity: rat. CAUTION: Please see the accession, sequence differs from that shown due to a frameshift in position 420. Other species not yet tested.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to the C-terminal of human ATG4C protein conjugated to Blue Carrier Protein. The peptide is homologous with the corresponding sequence derived from ATG4C protein in mouse and rat.
