GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE.
ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme).
Preferred substrate is MAP1LC3A.
Formation of the GABARAPL1-PE conjugate is essential for autophagy.
SUBUNIT: Interacts with ATG7 and ATG12.
The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5.
Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.
Belongs to the ATG3 family.
Applications:Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: 1:50-1:3000Immunofluorescence: 1:50-1:3000Western Blot: 1:50-1:3000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitute with 100ul of sterile ddH2O.
Specificity：Recognizes human ATG3 at ~37kD. Species Crossreactivity: rat and mouse
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to the central region of human ATG3, conjugated to Blue Carrier Protein. Cellular Localization: Cytoplasm. Species Sequence Homology: mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and Canis familiaris (canine).
