84-1099-01　β-Amyloid Protein Precursor, A4 (APP, ABPP, Abeta, APPI, A4) 100ul　A2275-85B

特徴

• Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis.
• Involved in cell mobility and transcription regulation through protein-protein interactions.
• Can promote transcription activation through binding to APBB1/Tip60 and inhibit Notch signaling through interaction with Numb.
• Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP.
• Inhibits G(o) alpha ATPase activity.
• Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1.
• May be involved in copper homeostasis/oxidative stress through copper ion reduction.
• Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV.
• Beta-amyloid peptides are lipophilic metal chelators with metal-reducing activity.
• Bind transient metals such as copper, zinc and iron.
• Rat and mouse beta-amyloid peptides bind only weakly transient metals and have little reducing activity due to substitutions of transient metal chelating residues.
• Beta-APP42 may activate mononuclear phagocytes in the brain and elicit inflammatory responses.
• Promotes both tau aggregation and TPK II-mediated phosphorylation (By similarity).
• The gamma-CTF peptides as well as the caspase-cleaved peptides, including C31, are potent enhancers of neuronal apoptosis.
• SUBUNIT: Binds, via its C-terminus, to the PID domain of several cytoplasmic proteins, including APBB family members, the APBA family, MAPK8IP1, SHC1, Numb and Dab1.
• Binding to Dab1 inhibits its serine phosphorylation.
• Also interacts with GPCR-like protein BPP, FPRL1, APPBP1, IB1, KNS2 (via its TPR domains), APPBP2 (via BaSS) and DDB1.
• In vitro, it binds MAPT via the MT-binding domains.
• Associates with microtubules in the presence of ATP and in a kinesin-dependent manner.
• Interacts, through a C-terminal domain, with GNAO1.
• Amyloid beta-42 binds CHRNA7 in hippocampal neurons.
• Beta-amyloid associates with HADH2.
• Tissue Specificity:different isoforms in different tissues: kidney.
• brain.
• liver.
• hippocampus, substania nigra pars compacta and cerebellum.
• In the cerebellum, all the isoforms are abundantly expressed in Purkinje cells.
• Applications:Suitable for use in Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.