Milk lipid globules from humans, cows and rats contain a protein identified as adipocyte differentiation-related protein (ADFP).
It is associated with the globule surface membrane material.
This protein, previously believed to be specific to adipocytes, is a major constituent of the globule surface and is present in a detergent-insoluble complex that contains butyrophilin and xanthine oxidase
ADFP (Adipophilin) is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells.
In tissues, however, expression of adipophilin is restricted to specific cell types, such as lactating mammary epithelial cells, adrenal cortex cells, Sertoli and Leydig cells of the male reproductive system, and steatosis or fatty change hepatocytes in alcoholic liver cirrhosis.
ADFP may be a possible new marker for the identification of specialized differentiated cells containing lipid droplets and for diseases associated with fat-accumulating cells.
Applications:Suitable for use in Western Blot, Immunofluorescence, Immunohistochemistry.
Staining was observed on the membrane of mouse liver cells.
Antigen retrievak with sodium citrate buffer, pH 6.0 is recommended prior to staining paraffin tissues
Immunofluorescence (IC): 1:50-1:200 Membrane staining was observed in HeLa cellsOptimal dilutions to be determined by the researcher.
Positive Control: Mouse and human liver lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 30% glycerol, 0.05% sodium azide.
Specificity：Recognizes mouse Adipophilin at ~51kD. Species Crossreactivity: human. Species sequence homology: rat, 88%.
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide made to the C-terminal region within residues 350-425 of the mouse protein (Swiss-Prot# P43883). Cellular Localization: Membrane.
