Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation and Supershift Assay.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Supershift: 0.5-1ul Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Purified
Purity:Purified by delipidation and immunoadsorption against an E. coli lysate immobilized on agarose beads.
Form:Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
Specificity:Recognizes human CREB-1, p43, MW:43kD. May react nonspecifically with other proteins. A partial crossreactivity is observed against CREM-1 protein. Species Crossreactivity: Human, mouse and rat
Isotype:IgG
Calc Applications Abbrev:E IP WB
Calc Crossreactivity:Hu Mo Rt
Immunogen:A synthetic peptide peptide corresponding to a region near the N-terminus (aa 5-45) of the human CREB-1 (p43)protein (KLH).