Interleukin-16, also known as lymphocyte chemoattractant factor (LCF), is a chemotactic cytokine that stimulates a migratory response in CD4+ lymphocytes, monocytes and eosinophils through binding to the CD4 receptor.
IL-16 induces human T-lymphocyte expression of interleukin 2 receptor and upregurates major histocompatibility complex molecules on resting human T lymphocyte.
It is able to render CD4+ T cells unresponsive to TCR/CD3 stimuli.
Moreover, IL-16 inhibits the replication of T cell and monocyte tropic HIV strains in vitro.
Applications:Suitable for use in Neutralization, ELISA and Western Blot.
Other applications not tseted.
Recommended Dilutions:Neutralization: 0.12-0.15ug/ml will yield one-half maximal inhibition [ND50] of the biological activity of hIL-16 (4.2ng/ml)
ELISA: ≥0.5ug/ml using 100ul/well antibody solution.
Allows the detection of 0.2-0.4ng/well of recombinant hIL-16
Western Blot: 0.1-0.2ug/ml The dectection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Specificity：Recognizes human IL-16.
Isotype：IgG
Calc Applications Abbrev：E N WB
Calc Crossreactivity：Hu
Immunogen：Highly pure recombinant human Interleukin-16
