Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells.
Disruption of this process has been implicated in a variety of diseases such as cancer (reviewed in 1).
The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death (reviewed in 2 and 3).
Bid, a pro-apoptotic Bcl-2 family member, is cleaved by caspase-8 in response to apoptotic signals (4,5), exposing the Bcl-2 homology 3 (BH3) domain which is normally buried in the full-length protein (6).
The cleaved complex is myristoylated and translocated to the mitochondrial membrane where it may induce mitochondrial Bax and Bak to oligomerize (7,8).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Store at -20°C or colder.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Highly Purified
Purity：Purified by Ion Exchange chromatography.
Form：Supplied as a liquid in PBS containing 0.02% sodium azide.
Specificity：Recognizes human Bid. Species Crossreactivity: Mouse.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Peptide corresponding to 14 amino acids near the middle of human Bid (GenBank accession no. AAH36364)
