84-1098-64　Secretory Leukocyte Protease Inhibitor (SLPI) 100ug　S0635

特徴

• Human Secretory Leukocyte Protease Inhibitor SLPI is a protein found in various secretory fluids, including parotid secretions, bronchial, nasal and cervical mucus, and seminal fluid.
• The protein is a potent inhibitor of a variety of proteolytic enzymes, including the neutrophil proteases, elastase and cathepsin G and the pancreatic proteases chymotrypsin and trypsin.
• SLPI is an important component of the antiprotease defense of tissues bathed by secretory fluids and could be useful as a therapeutic agent for treatment of the proteolytic damage of tissues seen in degenerative and inflammatory diseases.
• Applications:Suitable for use in Neutralization, Direct ELISA, Western Blot, and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Neutralization: Measured by its ability to neutralize Recombinant Human SLPI (0.125ug/ml) inhibition of Trypsin (0.05ug/mL) cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (10uM).
• The Neutralization Dose (ND50) is typically 2ug/ml
• Western Blot: 0.1ug/ml.
• Recombinant Human SLP1Immunohistochemistry: 5-15ug/ml using immersion fixed paraffin-embedded sections of human lungOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.