BID interacts with both Bcl2 and Bax through its BH3 domain.
It usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase-8 in response to TNF or Fas.
Cleaved BID, the C-terminal part of BID, translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis.
BID serves as a direct molecular link between caspase-8 activation and mitochondrial death machinery.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000.
At 0.5ug/ml, the dilution provides 100ml working solution, which at 10ml/blot allows 10 blots to be performed
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：10Blots
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in Dulbecco’s PBS (without Mg2+ and Ca2+), pH 7.2, 1% BSA, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes the 15kD fragment of mouse BH3 interacting domain death agonist (BID), a product of caspase-8 cleavage during apoptosis. It does not recognize the full length mouse BID or cleaved human BID. Species sequence homology: human (75%), rat (100%), chicken (62%).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to cleavage site (59/60) of mouse BID.
