Applications:Suitable for use in Western Blot, ELISA and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:ELISA: 1:5000-1:25,000Western Blot: 1:500-1:3000Immunohistochemistry: 1:500-1:1500Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder is stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O prior to use.
To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：2mg
Host：goat
Source Antibody：rat
Grade：Affinity Purified
Purity：Prepared from polyspecific antiserum by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% thimerosal. Reconstitute with 1ml sterile ddH2O. Labeled with Horseradish peroxidase (HRP).
Specificity：Recognizes rat IgG, IgA, IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase and anti-goat serum. Suitable for the detection of all rat immunoglobulin isotypes ad chain combinations.
