Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins.
Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis.
The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site.
Applications:Suitable for use in Immunoprecipitation, Dot Blot, ELISA, Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:ELISA: 1:20,000-1:100,000Western Blot: 1:2000-1:10,000Immunohistochemistry: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography followed by solid phase adsorption.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Glycerol-free.
Specificity：Recognizes mouse IgG, heavy and light chains. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, mouse IgG and mouse serum. Species Crossreactivity: Does not crossreact with human serum proteins.
