XCR1, also known as GPR5 and lymphotactin/SCM-1 (single cysteine motif 1) receptor, is a 38kD member of the G-protein coupled receptor 1 family.
It binds XCL1/lymphotactin/SCM-1α and XCL2/SCM-1beta.
In addition, human herpesvirus 8 (HHV8) encodes two viral chemokines vCCL2/vMIP-II and vCCL3/vMIP-III that function as an antagonist and a highly selective agonist, respectively, for XCR1.
XCR1 is expressed on neutrophils, CD8+ T cells , NK cells, CD4+ T cells and B cells.
Human XCR1 is a 333 amino acid (aa), 7-transmembrane molecule.
It contains a 32 aa N-terminus that lacks glycosylation sites and a 42 aa C-terminal cytoplasmic tail.
Over the extracellular regions used for immunization, human XCR1 shares 62%, 54% and 64% aa identity with canine, mouse and porcine XCR1, respectively.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 10ul labels 1x10e6 cellsOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：100Tests
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes human XCR1.
Isotype：IgG
Calc Applications Abbrev：FC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Thr31, Ser89-Lys103, His168-His190 and Phe251-Tyr267, joined with linker peptides from human XC Chemokine Receptor 1, extracellular N-terminus and loops, expressed in E. coli.
