VSIG2 (V-set and Ig domain-containing protein 2; also CTM and CT-like protein) is presumably a 50-60kD member of the CTX family of Ig-Superfamily proteins.
It shows expression in stomach and prostate by Northern blot, and likely participates in cell adhesion.
Mouse VSIG2 precursor is 328 amino acids (aa) in length.
It is a type I transmembrane (glyco)protein that contains a 220 aa extracellular domain (ECD) (aa 25-244) and a 63 aa cytoplasmic region.
The ECD contains one V-type (aa 25-138) and one C2-type Ig-like domain (aa 145-234).
Over aa 25-244, mouse VSIG2 is 94%, 83% and 85% aa identical to rat, canine and human VSIG2, respectively.
Two potential splice variants exist, one that shows a deletion of aa 305-328 and a second that shows a deletion of aa 144-236.
Applications:Suitable for use in Direct ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Direct ELISA: 0.5-1.0ug/ml detection limit for rmVSIG2 is ~ 1ng/well
Western Blot: 0.1-0.2ug/ml detection limit for rmVSIG2 is ~ 1 ng/lane under non-reducing and reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile 40-50% glycerol, PBS.
