84-1098-30　VSIG4 (V-set and Ig Domain-containing Protein 4, Z39Ig) 100ug　V4880

特徴

• VSIG4 (V-set and Ig domain-containing protein 4; also Z39Ig) is a 45-50kD macrophage-specific protein that belongs to the B7 family of molecules.
• Its constitutive expression depresses unwarranted T cell activation.
• Human VSIG4 is 399 amino acids (aa) in length.
• It is a type I transmembrane (TM) glycoprotein that contains a 264 aa extracellular domain (ECD) (aa 20-283) and a 95 aa cytoplasmic region.
• The ECD contains one V-type (aa 21-131) and one C2-type Ig-like domain (aa 143-226).
• Mouse VSIG4 has no C2-type domain.
• Over aa 20-139, human VSIG4 is 82% aa identical to mouse VSIG4.
• Multiple splice forms exist in human that impact the ECD and TM segments.
• One isoform deletes aa 67-143, while two additional isoforms substitute two aa’s for aa 138-233.
• Two new isoforms remove all or part of the TM region.
• Applications:Suitable for use in Direct ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:Direct ELISA: 0.5-1ug/mL detection limit for rhVSIG4 ~ 1ng/wellWestern Blot: 0.1-0.2ug/mL detection limit for rhVSIG4 ~ 1ng/lane under non-reducing and reducing conditions
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
• Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.