84-1098-29　Vitamin D Binding Protein (VDBP, VDB, DBP, DBP/GC, Gc Globulin, Group Specific Component, Group-specific Component, GC, hDBP, Vitamin D Binding alpha Globulin) 100ug　V2130-12

特徴

• VDBP (Vitamin D binding protein; also group-specific component and GC-globulin) is a 52-58kD, monomeric glycoprotein member of the ALB/AFP/VDB family of molecules.
• It is found in blood, urine and CSF, carries Vitamin D and its metabolites, and serves as an actin-scavenging protein.
• Mature mouse VDBP is 460 amino acids (aa) in length.
• It contains three albumin-type domains (aa 26-476) that are accompanied by 14 intrachain disulfide bonds.
• There are three potential alternative splice forms.
• One shows a deletion of aa 346-421, a second shows a 67 aa substitution for aa 345-421, and a third shows a 34 aa substitution for aa 346-423.
• All these variants involve the second and third albumin-like domains.
• Mature mouse VDBP (aa 17-476) is 77% and 90% aa identical to human and rat VDBP, respectively.
• Applications:Suitable for use in Direct ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.5ug/ml in lysates of mouse liverOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.