84-1098-28　VANGL2 (Vang-like 2 (van gogh, Drosophila) KIAA1215, LPP1, LTAP, MGC119403, MGC119404, STBM, Homolog of Drosophila Strabismus, Loop-tail-associated Protein, Vang (Van Gogh, Drosophila)-like 2, Vang (Van Gogh, Drosophila)-like 2, Vang, Van Gogh-like 2) 100ug　V0499-17B

特徴

• VANGL2 (van Gogh-like protein 2; also Strabismus and Ltap) is a 65kD member of the Vang family of proteins.
• It is found in the membranes of embryonic neurons and epithelium where it associates with DVL1-3 and various frizzleds.
• VANGL2 mutations are associated with neural tube defects.
• Human VANGL2 is a 521 amino acid (aa) 4-transmembrane protein that contains an N-terminal (aa 1-108) and C-terminal (aa 239-521) cytoplasmic domain.
• There is a Ser-rich region in the N-terminus (aa 5-20) and a PDZ-binding motif over aa 506-521.
• Over aa 241-521, human, mouse and rat show total identity in amino acid sequence.
• Applications:Suitable for use in Direct ELISA and Western Blo.
• Other applications not tested.
• Recommended Dilution:Direct ELISA: 0.5-1.0ug/ml detection limit for rhVANGL2 is ~ 1ng/well
• Western Blot: 1.0ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.