Human TRIM (T cell receptor–interacting molecule) (also trat1; T cell receptor-associated transmembrane adaptor 1, and pp29/30) is a 27-30kD, type III transmembrane protein, that is a member of the transmembrane adaptor protein (TRAP) family.
It contains a short, 8 amino acid (aa) extracellular region, a 19 aa transmembrane region, and a 159 aa cytosolic tail.
Its cytoplasmic tail contains several tyrosine motifs with the potential to bind to Src-homology 2 (SH2) domains of signaling proteins.
TRIM is present in T cells and NK cells.
Human TRIM shares 66% aa sequence identity with mouse TRIM.
Applications:Suitable for use in Flow Cytometry, Direct ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 25ug/ml use 10ul to label 10e6 cells.
Direct ELISA: 0.5-1.0ug/ml detection limit for rhTRIM is ~ 0.3ng/well
Western Blot: 1.0ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile 40-50% glycerol, PBS.
