Most cell types express three different sizes of TGF-Beta receptors.
These are designated Type I (53kD), Type II (70-85kD), and Type III (250-350kD).
The Type I receptor is a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-Beta.
The Type II receptor is also a membrane-bound serine/threonine kinase that binds TGF-Beta1 and TGF-Beta3 with high affinity and TGF-Beta2 with a much lower affinity.
The Type I and Type II receptors together form a heterodimeric signaling complex that is essential for the transduction of the anti-proliferative signals of TGF-Beta
The Type III receptor is a transmembrane proteoglycan with a large extracellular domain and a 43aa cytoplasmic domain.
The cytoplasmic domain of the Type III receptor lacks an obvious signaling motif and the receptor may not be involved directly in signal transduction.
The Type III receptor binds TGF-Beta2 with the highest affinity.
Other TGF-Beta isoforms also bind the Type III receptor, but with lower affinities.
Cellular responsiveness to TGF-Beta2 appears to be dependent on the presence of the Type III receptor which can interact with the signaling receptor complex.
In addition to the transmembrane Type III receptor, a soluble form of the receptor is secreted by some cell types.
The physiological role of this soluble receptor remains to be determined.
The recombinant TGF-Beta soluble receptor Type III binds the TGF-Beta isoforms differentially in solution and exhibits TGF-Beta antagonistic activities in vitro.
Applications:Suitable for use in ELISA, Western Blot and Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 10ul to label 10e6 human peripheral blood lymphocytes.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：100Tests
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes human TGF-Beta RIII. Species Crossreactivity: recombinant mouse TGF-Beta RIII; ~25%, recombinant human TGF-beta RI, recombinant human TGF-beta RII, recombinant human TGF-beta RIIB and recombinant mouse TGF-beta RI; <1%.
Isotype：IgG
Calc Applications Abbrev：E FC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gly21-Asp781 from the extracellular domain of human TGF-Beta R111, expressed in NS0 mouse cells. GenBank: AAA67061.
