Transforming Growth Factor beta Receptor III is a transmembrane proteoglycan that binds TGF- beta with high affinity.
Transforming Growth Factor beta Receptor III lacks a cytoplasmic signaling domain and functions as an accessory receptor that enhances efficient TGF-beta2 binding and signaling.
A soluble form of the receptor can be released by proteolytic cleavage of the membrane-bound form.
ApplicationsSuitable for use in Western Blot, Flow Cytometry and Direct ELISA.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1ug/ml using recombinant human Transforming Growth Factor beta Receptor IIIFlow Cytometry: 25ug/10e6 cells using PC-3 human prostate cancer cell lineOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：250ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 1.25ml sterile PBS.
Specificity：Recognizes human Transforming Growth Factor beta Receptor III. In direct ELISAs, ~25% cross-reactivity with recombinant mouse TGF-beta RIII is observed, and <1% cross-reactivity with recombinant human TGF-beta Receptor I, recombinant human TGF-beta Receptor II, recombinant human TGF-beta Receptor IIB, and recombinant mouse TGF-beta Receptor I is observed.
Isotype：IgG
Calc Applications Abbrev：E FC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gly21-Asp781 from human Transforming Growth Factor beta Receptor III, expressed in NS0 cells.
