ine protease inhibitor-clade B#6; also placental thrombin inhibitor) is a 42kD, cytosolic member of the ovalbumin (clade B)-subfamily, Serpin superfamily of protease inhibitors.
It is produced by multiple cell types (keratinocytes; monocytes; mast cells), and shows inhibitory activity towards thrombin, plasmin and chymotrypsin.
Human Serpin B6 is 376 amino acids (aa) in length.
An active inhibitor site lies between Glu327 and Met338.
Multiple potential isoforms exist.
One shows a 28 aa substitution for the N-terminal 191 amino acids.
Three others have alternate start sites that vary from 14-33 aa’s upstream of the standard start site.
Human Serpin B6 shares 76% aa identity with mouse Serpin B6.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.2ug/ml detection limit for rhSerpin B6 is ~ 5ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in Tris, pH 7.3, 1% BSA. Reconstitute with 1ml sterile 40-50% glycerol, Tris, 0.1mg/ml BSA. Labeled with Biotin.
