Ryk (related to tyrosine (Y) kinase; also VIK and MRK) is a member of the tyrosine protein kinase family.
It is a type I transmembrane glycoprotein that binds Wnt and forms a Wnt receptor complex with frizzled.
In this capacity, it serves as a link between Wnt and Dishevelled.
Mouse Ryk is 594 amino acids (aa) in length.
It contains a 177 aa extracellular region (aa 35-211) that shows one WIF-1-like domain (aa 51-180), and a cytoplasmic region that possesses a nonfunctional Ser/Thr protein kinase domain (aa 318-581).
There is one alternate start site at Met117.
Over aa 35-211, mouse Ryk is 94% aa identical to human Ryk, and shows absolute (100%) aa identity to rat Ryk.
Applications:Suitable for use in Direct ELISA, Immunocytochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:Direct ELISA: 0.5-1ug/ml.
The detection limit is ~1ng/well
Immunocytochemistry: 10ug/ml.
NIH3T3 cells were fixed with PBS, 4% paraformaldehyde and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA.
After blocking, cells were incubated with diluted R9951-50 followed by NL557-coupled anti-sheep IgG in the dark.
Between each step, cells were washed with PBS containing BSA
Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes mouse Ryk. Species Crossreactivity: human
