84-1098-14　Retinoic Acid Receptor Responder 1 (RARRES1, Tig1) 100ug　R1691-97

特徴

• RARRES1 (Retinoic Acid Receptor Responder 1; also Tig1) is a member of the protease inhibitor I47 family of proteins.
• It is expressed in epithelium, including keratinocytes, plus prostate and breast epithelium.
• It is considered to be a tumor-suppressor molecule, presumably acting as an inhibitor of carboxypeptidase A.
• Mouse RARRES1 is 349 amino acids (aa) in length.
• Based on human, it is a type II transmembrane (TM) protein with a TM segment at, or near, aa75-95.
• One potential 350aa splice variant exists that shows an almost complete aa divergence after Pro105.
• Applications:Suitable for use in Direct ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Immunohistochemistry (Fluorescence): 5-15ug/ml; Detects RARRES1 in cryosections of mouse liver.
• Tissue was fixed overnight in 4% PFA + 14% picric acid in PBS at 4°C.
• Sections were blocked with 10% donkey serum and 0.1% Triton X-100 in PBS, 1% BSA for 45 minutes at RT.
• After blocking, sections were incubated with R1691-97 for 3 hours at RT, followed by donkey anti-goat IgG (NL557) for 1 hour at RT.
• Between each step, cells were washed with PBS, 0.1% BSA
• Western Blot: 1ug/ml mouse spleen tissue lysatesOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.