84-1098-13　RAIDD (RIP-associated ICH-1/CED-3-homologous protein with DD, CRADD, Caspase and RIP adapter with DD) 100ug　R1032-03

特徴

• CRADD (Caspase and RIP adapter with death domain; also RAIDD) is a 22kD, widely-expressed cytosolic adaptor protein that participates in apoptosis.
• Mouse CRADD is 199 amino acids (aa) in length.
• It contains an N-terminal CARD region (aa 1-92) and a C-terminal DD/death domain (aa 116-188).
• The CARD interacts with caspase-2, while the DD recruits either PIDD or RIP.
• These interactions contribute to the activation of an apoptotic cascade.
• Mouse CRADD shows two potential additional isoforms.
• One shows an alternate start site at Met55, while a second shows a 70 aa substitution for aa’s 101-199.
• Mouse CRADD shares 90% and 95% aa identity with human and rat CRADD, respectively.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1.0ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.