Prohibitin 2 (PHB2; also B-cell receptor-associated protein BAP37 and REA) is a 37kD, ubiquitous member of the prohibitin family of molecules.
PHB2 is found in multiple intracellular compartments.
It is suggested to be a component of an inner mitochondrial membrane complex and a nuclear repressor of the estrogen receptor (ER).
Human PHB2 is 299 amino acids (aa) in length.
It contains a mitochondrial localization signal (aa 1-50), two ER repressor domains (aa 19-49 and aa 150-174), and a central coiled-coil domain (aa 190-238).
There may be one splice variant that shows a premature truncation after aa 289.
Over aa 89-299, mouse and rat PHB2 are absolutely (100%) identical to human PHB2.
Applications:Suitable for use in Direct ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Direct ELISA: 0.5-1.0ug/ml detection limit for rhPHB2 is ~ 6.5ng/well
Western Blot: 1.0ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile 40-50% glycerol, PBS.
