84-1098-09　PDL-2 (Programmed Cell Death 1 Ligand-2, Programmed Death Ligand 1, PD-L2, B7DC) 100ug　P3124-15E

特徴

• Applications:Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, Blocking of receptor-ligand interaction and Direct ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.2ug/mL with the appropriate secondary reagents to detect mouse PD-L2.
• The detection limit for rmPD-L2 is ~2ng/lane under non-reducing and reducing conditions.
• Immunohistochemistry: 2-15ug/ml will detect PD-L2 in cells and tissues.
• Flow Cytometry: on Tim-1 stimulated RAW 264.7 cells for use in flow cytometry.
• Dilute this antibody to 50ug/mL and add 10ul of the diluted solution to 1-2.5 x 10e5 cells in a total reaction volume not exceeding 200ul.
• The binding of unlabeled antibodies may be visualized by adding 10ul of a 25ug/mL stock solution of a secondary developing reagent such as anti-goat IgG conjugated to a fluorochrome.
• Blocking of receptor-ligand interaction: ~1-4ug/mL of this antibody will block 50% of the binding of 1ug/mL of rmPD-1 to immobilized rmPD-L2/Fc chimera (100ul of a 1ug/mL solution was coated in each well) in a functional ELISA.
• Direct ELISA: 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse PD-L2.
• The detection limit for rmPD-L2 is ~1.2 ng/well
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.