PBEF (pre-B cell colony-enhancing factor; also visfatin and nicotinamide phosphoribosyltransferase) is a 52kD member of the NAPRTase family of molecules.
It functions both intracellularly and extracellularly, where it participates in NAD synthesis and insulin receptor activation, respectively.
Human PBEF is 491 amino acids in length and contains no signal sequence.
There is at least one alternative splice form that shows a 5 aa substitution for the C-terminal 128 amino acids (aa 364-491).
Over aa 27-491, human PBEF shares 96%, 97% and 96% aa identity with mouse, porcine and canine PBEF, respectively.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.2ug/ml detection limit for rhPBEF is ~ 5ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in Tris, pH 7.3, 1% BSA. Reconstitute with 1ml sterile 40-50% glycerol, Tris, 0.1mg/ml BSA. Labeled with Biotin.
