PAM (Peptidyl-Gly alpha-amidating monooxygenase) is a dual-function, 95-110kD protein that belongs to both the lyase and monooxygenase enzyme families.
It is expressed in a variety of endocrine and exocrine glands and catalyzes the conversion of C-terminal Gly into amidated residues.
Human ProPAM is a 953aa type I transmembrane glycoprotein.
It contains an 833 aa extracellular domain (ECD) (aa31-863) and an 86 aa cytoplasmic tail.
The ECD shows one PHM/monooxygenase region (aa40-347) and a PAL/lyase domain (aa495-817).
Both regions are enzymatically active.
Alternative splicing may generate a soluble form (aa31-828), and remove aa388-494 that link the two embedded enzyme segments.
Over aa 1-817, human PAM is 91% aa identical to mouse PAM.
Applications:Suitable for use in Direct ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Direct ELISA: 0.5-1ug/ml.
The detection limit is ~0.2ng/well
Western Blot: 0.1-0.2ug/ml.
The detection limit is ~5ng/lane under non-reducing and reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human PAM.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to aa21-817 of human PAM isoform 1 expressed in NSO (P19021).
