Paired immunoglobulin-like, type 2 receptor beta (PILR-b) is a type I transmembrane (TM) glycoprotein belonging to the Ig superfamily.
It is the activating counterpart to the ITIM-bearing PILR-a inhibitory receptor.
PILR-b is expressed in a wide variety of tissues including hematopoietic cells.
Mature human PILR-b is a 208 amino acid (aa) protein with one V-type Ig-like extracellular domain, a truncated cytoplasmic tail, and positively-charged residues in its TM domain that interacts with ITAM-bearing adaptor molecules.
Within the V-type Ig-like region in their ECD, human PILR-b and PILR-a share a 92% aa sequence identity.
The aa sequence of mouse PILR-b ECD is only 43% identical to that of the human protein.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.2 ug/mL detection limit for rhPILR-b ~ 1ng/lane under non-reducing and reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from (PBS) containing 50ug of bovine serum albumin (BSA). Labeled with Biotin. Reconstitute with 200ul PBS.
Specificity：Recognizes human Paired lgG-Like Type 2 Receptor b.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：NS0-derived recombinant protein corresponding to human Paired lgG-Like Type 2 Receptor beta, Gln20-Ala189. Uniprot/Swiss Accession: Q9UKJ0
