NPTXR (Neuronal Pentraxin Receptor; also NPR) is a 65kD, type II transmembrane glycoprotein that binds Neuronal Pentraxins 1 and 2.
It is expressed on cerebellar Purkinje and granule cells, and hippocampal neurons of the CA1, CA3 and dentate gyrus regions.
Human NPTXR is 499 amino acids (aa) in length.
It contains a two aa cytoplasmic tail, a 21aa transmembrane segment, and a 476aa extracellular region with one Pentraxin domain (aa295-499).
One alternate start site exists at Met12, and a 55kD soluble form (possibly beginning at Gln56) may be generated by proteolytic cleavage.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1ug/mlImmunohistochemistry: 5-15ug/ml using immersion fixed paraffin-embedded sections of human brain (hippocampus)Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human NPTXR. Shows <5% crossreacitvity with rhNPTX1 and rhNPTX2 in direct ELISA.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ala24-Lys499 of human NPTXR, expressed in NS0 cells. Species Sequence Homology: mouse (87%)
