Notch-1 (so named for “notches” in fly wings; also TAN-1) is a 300kD member of the Notch family of glycoproteins.
It is associated with gene activation in both embryo and adult.
Human Notch-1 is a 2538 amino acid (aa) type I transmembrane glycoprotein.
It undergoes Golgi processing to generate a heterodimer composed of a 180-200kD disulfide-linked extracellular domain (aa 18-1664) and a 120kD membrane-bound segment (aa 1665-2556).
Upon ligand binding, the 110kD segment undergoes two cleavages which generate an NICD (notch intrImmunogen.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.2ug/mL detection limit for rhNotch-1 ICD ~ 0.5ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from (PBS) containing 50ug of bovine serum albumin (BSA). Labeled with Biotin. Reconstitute with 200ul 40-50% glycerol, PBS.
Specificity：Recognizes human Notch-1.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：E. coli-derived, recombinant human Notch-1 (aa 1754 - 2556)
