2B4, also known as CD244 and SLAMF4, is a 66kD type I transmembrane glycoprotein in the SLAM subgroup of the CD2 protein family.
SLAM family proteins have an extracellular domain (ECD) with two or four Ig-like domains and at least two cytoplasmic immunoreceptor tyrosine-based switch motifs (ITSMs).
2B4 interacts with CD48, while other SLAM family proteins interact homophilically.
Mature human 2B4 consists of a 208 aa ECD with two Ig-like domains, a 21 aa transmembrane segment, and a 120 aa cytoplasmic domain with four ITSMs.
Three additional splice variants of human 2B4 have deletions of the short region between the Ig-like domains, the second Ig-like domain, or a portion of the cytoplasmic tail.
Within the ECD, human 2B4 shares 46% and 40% aa sequence identity with mouse and rat 2B4, respectively.
The ECD of human 2B4 shares 17%-24% aa sequence identity with comparable regions of human CD2 family members BLAME, CD2F-10, CD84, CD229, CRACC, NTB-A, and SLAM.
2B4 is expressed on all NK cells, γδ T cells, monocytes, some CD4+ and CD8+ T cells, and some dendritic cells.
CD48 mediates 2B4+ cell interactions with nearly all hematopoietic cell types, including cells of the same type.
2B4/CD48 signaling cooperates with other receptor systems to either promote or inhibit NK and CD8+ T cell activation.
The inhibitory activities are distinct from those of MHC I restricted inhibitory NK cell receptors.
Ligation of 2B4 with antibodies or CD48 constructs can either directly trigger inhibitory signaling or disrupt an inhibitory interaction, leading to cellular activation.
The inhibitory effect is associated with the long form of 2B4, while the activation is associated with the short form.
2B4 can also induce signaling through CD48.
Applications:Direct ELISA, Western Blot, Flow Cytometry, Immunohistochemistry
Recommended Dilution:Direct ELISA: This antibody can be used at 0.5-1.0ug/mL with the appropriate secondary reagents to detect mouse 2B4.
The detection limit for rm2B4 is approximately 0.3 ng/well.
In this format, this antibody shows approximately 5% cross-reactivity with rh2B4 and less than 1% cross-reactivity with rhSLAM.
Western Blot: This antibody can be used at 0.1-0.2ug/mL with the appropriate secondary reagents to detect mouse 2B4.
The detection limit for rm2B4 is approximately 5 ng/lane under non-reducing and reducing conditions.
Flow Cytometry: This antibody can be used at 3-10ug/mL/10e6 cells with an appropriate secondary antibody for indirect immunofluorescence staining of cells by flow cytometry.
Immunohistochemistry: This antibody will detect 2B4 in cells and tissues.
The working dilution is 5-15ug/mL.
Optimal dilutions to be determined by the researcher.
Endotoxin:≤ 0.01 EU /1ug (LAL)
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitution: Reconstitute with sterile PBS. If 1ml of PBS is used, the antibody concentration will be 500ug/ml.
