Produced in goats immunized with purified, NS0-derived, recombinant human Nectin-3 (rhNectin-3; aa 56-400; Accession # AAF97597) extracellular domain.
Human Nectin-3 specific IgG was purified by human Nectin-3 affinity chromatography.
Nectin-3, also known as poliovirus receptor-related 3, is a 95kD type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins.
The extracellular domains of the Nectin family form cis-homodimers that are homophilic and heterophilic trans-interacting cell adhesion molecules, which play important roles in various cell-cell junctions.
Human Nectin-3 extracellular domain shares 94% amino acid sequence identity with that of mouse Nectin-3 and then biotinylated.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.2ug/ml detection limit for rhNectin-3 is ~ 2ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in Tris, pH 7.3, 1% BSA. Reconstitute with 1ml sterile 40-50% glycerol, Tris, 0.1mg/ml BSA. Labeled with Biotin.
