NAALADL2 (N-acetylated a-linked acidic dipeptidase like 2) is a member of the peptidase M28 family of enzymes.
It is presumably a type II transmembrane (TM) protein that may have O-glycosyl hydrolase activity.
Human NAALADL2 is 795 amino acids (aa) in length.
It is believed to contain a cytoplasmic and TM segment at the N-terminus, followed by a peptidase domain (aa 444-596) and a TfR-like dimerization region (aa 688-777).
There are multiple splice forms.
One shows a 4 aa substitution for aa 292-795, a second shows an alternate start site at Met18 with a 24 aa substitution for aa 314-795, and a third contains an alternate start site at human NAADADL2 is 87% and 82% aa identical to mouse and canine NAADADL2, respectively.
Applications:Suitable for use in Flow Cytometry, Direct ELISA, Western Blot, Immunocytochemistry and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Flow Cytometry: use 10ul to label 10e6 cells
Direct ELISA: 0.5 -1ug/mL detection limit for rhNAALADL2 ~ 0.3ng/well
Western Blot: 0.1-0.2ug/ml detection limit for rhNAALADL2 ~ 10ng/lane under non-reducing and reducing conditions
Immunocytochemistry: 10ug/mLOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS with 5% trehalose. Reconstitute with 200ul 40-50% glycerol, PBS.
Specificity：Recognizes human NAALADL2.
Isotype：IgG
Calc Applications Abbrev：E FC IC IP WB
Calc Crossreactivity：Hu
Immunogen：NS0-derived, recombinant human NAALADL2. (aa 152 - 795)
