84-1097-92　Mrc2 (Macrophage Mannose Receptor 2, uPARAP, Endo180) 100ug　M4685-94

特徴

• Mrc2 (macrophage mannose receptor 2; also uPARAP/Endo180) is a 180kD member of the mannose receptor family.
• It is found on fibroblasts, macrophages and endothelial cells.
• Mrc2 binds and internalizes glycoproteins plus collagen types I and IV.
• Mouse Mrc2 is a type I transmembrane glycoprotein that is 1479 amino acids (aa) in length.
• It possesses a 1383 aa extracellular domain (ECD) (aa 31-1479) and a short 45 aa cytoplasmic tail.
• The ECD contains eight C-type lectin domains plus one fibronectin type II domain (aa 181-229) that binds collagen.
• There are two splice variants, one which shows an eight aa substitution for aa 173-1479, and a second that shows a 72 aa substitution for aa 982-1479.
• Over aa 31-1413, mouse Mrc2 shares 91% and 97% aa identity with human and rat Mrc2, respectively.
• Applications:Suitable for use in Direct ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:Direct ELISA: 0.5-1.0ug/ml detection limit for rmMrc2 is ~ 1ng/well
• Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.